Elimination of tumorigenic stem cells from differentiated progeny and selection of definitive endoderm reveals a Pdx1+ foregut endoderm stem cell lineage
نویسندگان
چکیده
منابع مشابه
stauprimide priming of human embryonic stem cells toward definitive endoderm
objective: in vitro production of a definitive endoderm (de) is an important issue in stem cell-related differentiation studies and it can assist with the production of more efficient endoderm derivatives for therapeutic applications. despite tremendous progress in de differentiation of human embryonic stem cells (hescs), researchers have yet to discover universal, efficient and cost-effective ...
متن کاملDevelopment of definitive endoderm from embryonic stem cells in culture.
The cellular and molecular events regulating the induction and tissue-specific differentiation of endoderm are central to our understanding of the development and function of many organ systems. To define and characterize key components in this process, we have investigated the potential of embryonic stem (ES) cells to generate endoderm following their differentiation to embryoid bodies (EBs) i...
متن کاملStauprimide Priming of Human Embryonic Stem Cells toward Definitive Endoderm
OBJECTIVE In vitro production of a definitive endoderm (DE) is an important issue in stem cell-related differentiation studies and it can assist with the production of more efficient endoderm derivatives for therapeutic applications. Despite tremendous progress in DE differentiation of human embryonic stem cells (hESCs), researchers have yet to discover universal, efficient and cost-effective p...
متن کاملMild electrical stimulation with heat shock guides differentiation of embryonic stem cells into Pdx1-expressing cells within the definitive endoderm
BACKGROUND Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrine β cells. Mild electrical stimulation was reported to modulate the differentiation of ES cells into ectoderm-derived neuronal cells or mesoderm-derived cardiac cells. RESULTS...
متن کاملPolycomb enables primitive endoderm lineage priming in embryonic stem cells
Mouse embryonic stem cells (ESCs), like the blastocyst from which they are derived, contain precursors of the epiblast (Epi) and primitive endoderm (PrEn) lineages. While transient in vivo, these precursor populations readily interconvert in vitro. We show that altered transcription is the driver of these coordinated changes, known as lineage priming, in a process that exploits novel polycomb a...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2011
ISSN: 1873-5061
DOI: 10.1016/j.scr.2010.10.003